人才队伍
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  研究员
 
姓名  
柏文琴
性别  
专家类别  
N/A
职称  
研究员
学历  
博士研究生
电话  
022-84861999
传真  
N/A
电子邮件  
baiwq@tib.cas.cn
地址  
天津市空港经济区西七道32号
邮编  
300308

简历

2010-2013年,中国科学院微生物研究所,博士

2014-2015年,中国科学院天津工业生物技术研究所,助理研究员

2015-2019年,明尼苏达大学和圣路易斯华盛顿大学,访问学者,博士后

2020-至今,中国科学院天津工业生物技术研究所,研究员,博士生导师


研究方向:
  

主要利用合成生物学和代谢工程技术,围绕重要工业酶、医用蛋白及活性聚合物等生物大分子的微生物合成开展基础及应用研究。


承担科研项目情况:
  

国家重点研发计划绿色生物制造项目,重要氨基酸工业菌种系统改造与产业示范,2021.01-2024.12,课题负责人

国家自然科学青年基金项目,11家族木聚糖酶嗜碱机制研究,2015.01-2017.12,主持

中国科学院国际合作项目,基于耐热多形汉逊酵母建立蛋白材料合成新型平台,2023.01-2025.12,项目负责人

天津市合成生物技术创新能力提升行动,基于丝蛋白的功能材料的设计与合成,2020.06-2023.05,项目负责人

天津市合成生物技术创新能力提升行动,蛋白酶资源库创建与功能表征,2021.10-2024.09,任务负责人


获奖及荣誉:
  


代表论著:

Nature CommunicationsMetabolic EngineeringCarbohydrate PolymersMicrobial Cell Factories等期刊发表论文30余篇,申请专利17项,授权7项,成果转化2项。

1. Sijia Wang†, Wenxin Huang†, Zhaoxuan Feng, Xiaoli Tian, Dexin Wang, Lang Rao, Ming Tan, Niran Roongsawang, Hui Song*, Wenxia Jiang*, Wenqin Bai*. Laccase-mediated formation of hydrogels based on silk-elastin-like protein polymers with ultra-high molecular weight. International Journal of Biological Macromolecules. 2023, 231:123239. DOI: 10.1016/j.ijbiomac.2023.123239. (IF 8.2)

2. Shuyu Chen†, Hongchen Zheng†, Jiaqi Gao, Hui Song, Wenqin Bai*. High-level production of pullulan and its biosynthesis regulation in Aureobasidium pullulans BL06. Frontiers in Bioengineering and Biotechnology. 2023, DOI:10.3389/fbioe.2023.1131875. (IF 5.7)

3. Xi Sun, Jun Yang, Xiaoping Fu, Xingya Zhao, Jie Zhen, Hui Song, Jianyong Xu, Hongchen Zheng*, Wenqin Bai*. Trehalose Production Using Three Extracellular Enzymes Produced via One-Step Fermentation of an Engineered Bacillus subtilis Strain. Bioengineering-Basel. 2023 Aug 18; 10(8):977. Doi: 10.3390/bioengineering10080977. (IF 4.6)

4. Yifan Yang, Xiaoping Fu, Xingya Zhao, Jianyong Xu, Yihan Liu*, Hongchen Zheng*, Wenqin Bai*, Hui Song. Overexpression of a thermostable α-amylase through genome integration in Bacillus subtilis. Fermentation-Basel. 2023, 9(2):139. https://doi.org/10.3390/fermentation9020139. (IF 3.7)

5. Zhaoxuan Feng†, Sijia Wang†, Wenxin Huang†, Wenqin Bai*. A potential bilayer skin substitute based on electrospun silk-elastin-like protein nanofiber membrane covered with bacterial cellulose. Colloids and Surfaces B: Biointerfaces. Accept. (IF 5.8)

6. Dexin Wang, Xiaoping Fu, Dasen Zhou, Jiaqi Gao, Wenqin Bai*. Engineering of a newly isolated Bacillus tequilensis BL01 for poly-γ- glutamic acid production from citric acid. Microbial Cell Factories. 2022, 21(1):276. DOI: 10.1186/s12934-022-01994-z. (IF 6.4)

7. Zhaoxuan Feng, Jinsong Li, Dasen Zhou, Hui Song, Jiaqi Lv*, Wenqin Bai*. A novel photocurable pullulan-based bioink for digital light processing 3D printing. International Journal of Bioprinting. 2022, 9(2). DOI: 10.18063/ijb.v9i2.657. (IF 8.4)

8. Zhaoxuan Feng†, Shuyu Chen†, Abdullah Ahmad, Longjiao Chen, Wenqin Bai*. Ultra-high molecular weight pullulan-based material with high deformability and shape-memory properties. Carbohydrate Polymers. 2022, 295:119836. DOI: 10.1016/j.carbpol.2022.119836. (IF 11.2)

9. Wenqin Bai†, Winston E. Anthony†, Christopher Hartline, Shaojie Wang, Bin Wang, Jie Ning, Fong-Fu Hsu, Gautam Dantas*, Fuzhong Zhang*. Engineering Diverse Fatty Acid Compositions of Phospholipids in Escherichia coli. Metabolic Engineering. 2022, 74:11-23. DOI: 10.1016/j.ymben.2022.08.011. (IF 8.4)

10. Wenqin Bai, Cameron J. Sargent, Jeong-Mo Choi, Rohit V. Pappu, Fuzhong Zhang*. Covalently-assembled single-chain protein nanostructures with ultra-high stability. Nature Communications. 2019, 10, 3317. doi://doi.org/10.1038/s41467-019-11285-8. (IF 16.6)

11. Wenqin Bai, Weitao Geng, Shaojie Wang and Fuzhong Zhang*. Biosynthesis, regulation, and engineering of microbially produced branched biofuels. Biotechnology for Biofuels. 2019, 12:84. (IF 6.3)

12. Christopher H. Bowen, Bin Dai, Cameron J. Sargent, Wenqin Bai, Pranay Ladiwala, Huibao Feng, Wenwen Huang, David L. Kaplan, Jonathan M. Galazka, Fuzhong Zhang*. Recombinant spidroins fully replicate primary mechanical properties of natural spider silk. Biomacromolecules, 2018, 19, 3853-3860. (IF 6.2)

13. Jingyu Wang, Jilong Wang, Yi-shu Tai, Qiuge Zhang, Wenqin Bai, Kechun Zhang*. Rerouting carbon flux for optimized biosynthesis of mesaconate in Escherichia coli. Applied Microbiology and Biotechnology. 2018, 102(17): 7377-7388. (IF 5.2)

14. Wenqin Bai, Yi-Shu Tai, Jingyu Wang, Jilong Wang, Pooja Jambunathan, Kevin J. Fox, Kechun Zhang*. Engineering Nonphosphorylative Metabolism to Synthesize Mesaconate from D-Xylose and L-Arabinose in Escherichia coli. Metabolic Engineering, 2016 (38): 285–292. (IF 8.4)

15. Wenqin Bai*, Yufan Cao, Jun Liu, Qinhong Wang, Zhenhu Jia. Improvement of alkalophilicity of an alkaline xylanase Xyn11A-LC from Bacillus sp. SN5 by random mutation and Glu135 saturation mutagenesis. BMC Biotechnology, 2016(16):77 (IF 3.5)

16. Jilong Wang, Suthamat Niyompanich, Yi-Shu Tai, Jingyu Wang, Wenqin Bai, Prithviraj Mahida, Tuo Gao and Kechun Zhang*. Engineering Highly Efficient E. coli strain for Mevalonate Fermentation by Chromosomal Integration. Applied and Environmental Microbiology, 2016, 82(24):7176-7184. (IF 4.4)

17. Wenqin Bai, Yanfen Xue, Yueju Zhao, Qinhong Wang, Yanhe Ma*. Structural insight into and mutational analysis of family 11 xylanases: implications for mechanisms of higher pH catalytic adaptation. PLoS One. 2015, Jul 10, 10(7):e0132834. (IF 3.7)

18. Wenqin Bai, Yanfen Xue, Cheng Zhou, Yanhe Ma*. Cloning, expression and characterization of an alkali-tolerant xylanase from alkalophilic Bacillus sp. SN5. Biotechnol Appl Biochem. 2015, 62(2):208-17. (IF 2.8)

19. Wenqin Bai, Cheng Zhou, Yanfen Xue, Chunhsiang Huang, Ruiting Guo, Yanhe Ma*. Three-dimensional structure of an alkaline xylanase Xyn11A-LC from alkalophilic Bacillus sp. SN5 and improvement of its thermal performance by introducing arginines substitutions. Biotechnol Lett. 2014, 36(7):1495-501. (IF 2.7)

20. Wenqin Bai, Yanfen Xue, Cheng Zhou, Yanhe Ma*. Cloning, expression and characterization of a novel salt-tolerant xylanase from Bacillus sp. SN5. Biotechnol Lett. 2012, 34(11):2093-9. (IF 2.7)